Journal: BMC Genomics
Article Title: The FU gene and its possible protein isoforms
doi: 10.1186/1471-2164-5-49
Figure Lengend Snippet: Analyses of alternative splicing by nested PCR . Nested PCR was performed on cDNA from 8 tissues, over regions implicated to undergo alternative splicing. These are encoded by exons 8 (panel A), 13 (panel B) and 24 (panel C). Available cDNA clones either with or without these parts served as controls as indicated. The PCR products were analyzed on agarose gels as shown.
Article Snippet: The expression of exon 8, 13 and 24 sequences in mRNA was assessed by nested PCR on RT/PCR generated cDNA samples from eight different tissues as provided in Human Multi Tissue cDNA Panel II (Clontech).
Techniques: Nested PCR, Clone Assay